Dosage protocol · Longevity & Mitochondrial Health
NAD+ 1000 mg
Typical daily range: 50–100 mg once daily subcutaneously (gradual titration from lower doses).
- Reconstitute: Add 3.0 mL bacteriostatic water → 333.3 mg/mL concentration.
- Typical daily range: 50–100 mg once daily subcutaneously (gradual titration from lower doses).
- Easy measuring: At 333.3 mg/mL, 1 unit = 0.01 mL = 3.33 mg on a U‑100 insulin syringe.
- Storage: Lyophilized: freeze at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F) for up to 14 days; protect from light and avoid freeze–thaw cycles.
BAC water3.0mL
Concentration333.3mg/mL
Reconstitution
NAD+ dosage chart
NAD+ dosage chart
| Week | Dose | Draw |
|---|---|---|
| Week 1 | 50 mg | 15 units0.15 mL |
| Week 2 | 75 mg | 22.5 units0.225 mL |
| Weeks 3–8 | 100 mg | 30 units0.30 mL |
| Weeks 9–12 | 100 mg | 30 units0.30 mL |
| Weeks 13–16 | 100 mg | 30 units0.30 mL |
How much to draw
The Draw column shows exactly where to stop on a U-100 insulin syringe. Different vial size or dose? The calculator below recomputes it live.
Supplies needed
Supplies needed
Peptide vial
The lyophilized compound
Bacteriostatic water
For reconstitution
U-100 insulin syringes
One per injection
Alcohol swabs
Stopper + site each time
How it works
Despite being searched and marketed as "NAD+ peptide," NAD+ (nicotinamide adenine dinucleotide) is not a peptide at all — it contains no amino-acid chain. It is a small nucleotide-derived coenzyme, built from a vitamin-B3-related nicotinamide group, adenine, and sugar-phosphate units, present in every cell in the body.[1]
It exists in oxidized (NAD+) and reduced (NADH) forms that shuttle electrons between metabolic reactions. The "peptide" label mainly reflects wellness-clinic marketing conventions that group it alongside actual peptide therapies, not any chemical similarity to one.
Benefits & side effects
Human trials of oral NR have shown it is bioavailable and raises NAD+ metabolites, and one randomized trial found NMN improved a measure of muscle insulin sensitivity in prediabetic women.[8] Results for symptom-level outcomes — fatigue, cognition, physical function — are mixed across studies, and a rise in a biomarker does not automatically translate into a proven clinical benefit.
Evidence for IV or injectable NAD+ addressing wellness, anti-aging, cognitive, or addiction-recovery claims is essentially absent at the level of controlled clinical trials; those specific claims currently rest on mechanistic plausibility and marketing rather than trial data.
Calculator
Plan your own dose
Reconstituting a different vial size or targeting a different dose? Run the numbers.
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