Dosage protocol · Healing & Recovery
TB-500 10 mg
Typical daily range: 500–1000 mcg once daily (gradual titration recommended).
- Reconstitute: Add 3.0 mL bacteriostatic water → ~3.33 mg/mL concentration.
- Typical daily range: 500–1000 mcg once daily (gradual titration recommended).
- Easy measuring: At 3.33 mg/mL, 1 unit = 0.01 mL ≈ 33.3 mcg on a U‑100 insulin syringe.
- Storage: Lyophilized: store at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F); do not freeze reconstituted solution.
BAC water3.0mL
Concentration3.33mg/mL
Reconstitution
TB-500 dosage chart
TB-500 dosage chart
| Phase | Dose | Draw |
|---|---|---|
| Weeks 1–2 | 500 mcg | 15 units0.15 mL |
| Weeks 3–4 | 600 mcg | 18 units0.18 mL |
| Weeks 5–8 | 750 mcg | 23 units0.23 mL |
| Weeks 9–12 | 1000 mcg | 30 units0.30 mL |
How much to draw
The Draw column shows exactly where to stop on a U-100 insulin syringe. Different vial size or dose? The calculator below recomputes it live.
Supplies needed
Supplies needed
Peptide vial
The lyophilized compound
Bacteriostatic water
For reconstitution
U-100 insulin syringes
One per injection
Alcohol swabs
Stopper + site each time
How it works
TB-500 is commonly described as a synthetic peptide related to thymosin beta-4, a naturally occurring 43-amino-acid actin-binding protein found throughout the body and studied for roles in cell movement, wound repair, and tissue remodeling.[1] "TB-500" itself is not a standardized, FDA-recognized drug name — it is an informal, unregulated label, so products sold under it can vary in identity and quality even when marketed similarly.
TB-500 has no history as an approved drug at any point. It is sold exclusively as an unapproved research chemical, and most of what is known about its proposed biology comes from research on thymosin beta-4 itself rather than on TB-500-specific human trials.
Benefits & side effects
Direct TB-500 evidence is thin. Most supporting data comes from thymosin beta-4 research: animal wound-healing studies reported accelerated repair, and separate translational work examined thymosin beta-4's role in cardiac cell migration and survival after injury.[4] Registered human trials of thymosin beta-4-related investigational products exist for a small number of indications, such as ocular surface conditions, but these involve specific formulations that are not necessarily equivalent to TB-500 products sold online.[5]
Claims about faster tendon or ligament recovery, reduced inflammation, or "Wolverine-like" healing rely on extrapolating from this preclinical and indirect human evidence rather than on controlled trials of TB-500 itself, and should be treated as unproven hypotheses rather than established outcomes.
Calculator
Plan your own dose
Reconstituting a different vial size or targeting a different dose? Run the numbers.
1
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